DNA Electrophoresis Results
Detection of Sickle Cell Anaemia
Analysing Results.
“This DNA Electrophoresis experiment is a Punnet Square in Action!"
In the picture of this gel we have 3 controls points. Lane 1 is homozygous recessive for the mutated Beta Haemoglobin gene, causing sickle cell disease. Lane 2 is heterzygous meaning they have one copy of the normal Allele for the Beta Haemoglobin Gene and one copy of the Alllele for Beta Haemoglobin that codes for Sickle Cell Anemia and hence is a carrier. Lane 3 is homozygous recessive which means they have 2 copies of the normal allele for Beta Haemoglobin so the individual does not have the disease.
The mother is a carrier and has the genotype Ss - carrier, the child is has the genotype ss - has the sickle cell disease (unfortunately) and then finally the father is a carrier and has the genotype Ss.
Interpreting Data.
When you use the data we have derived from the gel in a punnet square we can see that any offspring have a 25% chance of having the Sickle Cell Disease.